Plant Transcription Factor Database
Previous version: v3.0
Fragaria x ananassa
HSF Family
Species TF ID Description
FANhyb_icon00001976_a.1.g00001.1HSF family protein
FANhyb_icon00008414_a.1.g00001.1HSF family protein
FANhyb_icon00011864_a.1.g00001.1HSF family protein
FANhyb_icon00012528_a.1.g00001.1HSF family protein
FANhyb_icon00034214_a.1.g00001.1HSF family protein
FANhyb_icon00035201_a.1.g00001.1HSF family protein
FANhyb_icon00049029_a.1.g00001.1HSF family protein
FANhyb_rscf00000005.1.g00037.1HSF family protein
FANhyb_rscf00000041.1.g00023.1HSF family protein
FANhyb_rscf00000382.1.g00003.1HSF family protein
FANhyb_rscf00000407.1.g00004.1HSF family protein
FANhyb_rscf00000416.1.g00010.1HSF family protein
FANhyb_rscf00000797.1.g00003.1HSF family protein
FANhyb_rscf00001483.1.g00001.1HSF family protein
FANhyb_rscf00001679.1.g00003.1HSF family protein
FANhyb_rscf00002773.1.g00001.1HSF family protein
FANhyb_rscf00003403.1.g00002.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645